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Save my name and email in this browser for the next time I comment. Principle of Methyl Red MR Test Some bacteria have the ability to utilize glucose and convert it to a stable acid like lactic acid, acetic acid or formic acid as the end product.

Using organisms taken from an hour pure culture, lightly inoculate the medium. Incubate aerobically at 37 degrees C.

Following 24 hours of incubation, aliquot 1ml of the broth to a clean test tube. Reincubate the remaining broth for an additional 24 hours. Add 2 to 3 drops of methyl red indicator to aliquot.

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